In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.
نویسندگان
چکیده
An RNA-DNA copolymer was isolated from Escherichia coli infected with bacteriophage T4. The RNA and DNA are covalently linked, and in the same polynucleotide strand. The DNA of the copolymer hybridizes specifically to the left strand of phage T4 DNA. The copolymer is produced in cells infected with amber mutants of phage T4 deficient in DNA replication and is not inhibited by the addition of chloramphenicol.
منابع مشابه
Inhibition of host protein synthesis during infection of Escherichia coli by bacteriophage T4. I. Continued synthesis of host ribonucleic acid.
The ribonucleic acid (RNA) synthesized at specified intervals during infection of Escherichia coli K-12 by bacteriophage T4 was hybridized to denatured E. coli or T4 deoxyribonucleic acids (DNA). The reactions were performed under conditions that maximized the yield and at RNA/DNA inputs such that excess DNA sites were available for all RNA species. Most of the RNA synthesized at any time durin...
متن کاملControl of early gene expression of bacteriophage T4: involvement of the host rho factor and the mot gene of the bacteriophage.
Many early mRNA species of bacteriophage T4 are not synthesized after infection of Escherichia coli in the presence of chloramphenicol. This has been interpreted as a need for T4 protein(s) to be synthesized to allow expression of some early genes, e.g., those for deoxycytidinetriphosphatase, deoxynucleosidemonophosphate kinase and UDP-glucose-DNA beta-glucosyltransferase. In the experiments de...
متن کاملNuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant unable to induce endonuclease II.
Nuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant deficient in the ability to induce endonuclease II indicates that either (i) the endonuclease II-catalyzed reaction is not the first step in host deoxyribonucleic acid (DNA) breakdown or (ii) nuclear disruption is independent of nucleolytic cleavage of the host chromosome. M-band analysis demonstrates that the...
متن کاملChemical structure of a modification of the Escherichia coli ribonucleic acid polymerase alpha polypeptides induced by bacteriophage T4 infection.
The cr-polypeptides of Escherichia coli RNA polymerase are known to be chemically modified within 4 min after infection by bacteriophage Tq. This paper reports a crude system derived from T1-infected E. coli which will modify the cr-polypeptides of purified E. coli RNA polymerase in vitro. The product of this in vitro reaction is identical with the modified (Y found in viva ; it differs chemica...
متن کاملInhibition of host protein synthesis during infection of Escherichia coli by bacteriophage T4. II. Induction of host messenger ribonucleic acid and its exclusion from polysomes.
Two gene clusters on the Escherichia coli chromosome were induced at early times after T4 infection when >99% of the cells were infected: the lactose (lac) operon and prophage lambda. Their messenger ribonucleic acid (mRNA) was detected by hybridization to phi80 dlac deoxyribonucleic acid (DNA) and lambdaDNA, respectively. Synthesis of host mRNA could be initiated during the first few minutes a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 69 11 شماره
صفحات -
تاریخ انتشار 1972