Relationship between Periodontal Inflamed Surface Area [PISA] and anaerobic periodontal infections assessed by BANA [N-benzoyl-DL- arginine-β-napthylamide] assay

Background: chronic periodontitis is an inflammatory disease of the periodontium caused by microbial infection. The BANA test is a simple chairside test which allows the clinician to quantify the amount of anaerobic periodontal infection. PISA quantifies the inflammatory burden posed by periodontitis to cause systemic inflammatory response. By comparing the BANA test values with PISA, the validity of PISA as a tool to quantify the amount of inflamed periodontal tissues can be analysed. Aim: to compare PISA values with anaerobic periodontal infection assessed by BANA assay. Patients and Methods: A total of 80 sites were selected [40 each from 10 patients in healthy group and 10 patients in chronic periodontitis group]; after measuring the probing depth/clinical attachment levels, the tooth with deepest probing pocket depth from each sextant was selected, and the plaque sample was collected for BANA assay and the corresponding PISA values were calculated. Results: The Mann-Whitney test was used to compare the BANA test results with PISA values in healthy and periodontitis groups. There was a significant difference [p≤0.0001], in PISA values from BANA positive sites compared to BANA negative sites in both the groups. Conclusion: PISA values can be considered as indicators of anaerobic periodontal infection, which clearly demonstrates the validity of PISA in quantifying the inflammatory burden.