Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli.
نویسندگان
چکیده
A cDNA clone coding for the major house dust mite allergen Der p 1 was isolated from a lambda gt 11 library. Its sequence correlates with known amino acid sequences of Der p 1 and it produces a fusion protein which reacts with rabbit anti-Der p 1 antiserum.
منابع مشابه
Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli.
Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, th...
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ورودعنوان ژورنال:
- Agricultural and biological chemistry
دوره 55 5 شماره
صفحات -
تاریخ انتشار 1988