Expression in sugar beet of the introduced cercosporin toxin export (CFP) gene from <Emphasis Type="Italic">Cercospora kikuchii</Emphasis>, the causative organism of purple seed stain in soybean
نویسندگان
چکیده
The Cercospora kikuchii cercosporin export gene, CFP, introduced into Beta vulgaris L. by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene. Transcriptional expression of the CFP gene in leaves was determined by RT-PCR using CFP-specific primers. CFP protein was detected using Western analysis with an affinity-purified polypeptidespecifc antibody. Analysis of the relative susceptibility of CFP-transgenic and non-transgenic sugar beet plants is planned but will probably take several years to complete.
منابع مشابه
More Cercospora Species Infect Soybeans across the Americas than Meets the Eye
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf ...
متن کاملSoybean Seed Health
Fig. 3. Wrinkled, white, moldy-appearing seed infected by Phomopsis spp. More than 30 fungi are seed-borne on soybeans. Some, such as Phomopsis spp., the cause of pod and stem blight disease, can severely reduce seed viability (fi g. 1). Others cause quality problems, such as purple seed stain, caused by Cercospora kikuchii, but have less severe effects on viability. However, most fungi that ar...
متن کاملGenetic mapping of resistance to purple seed stain in PI 80837 soybean.
Purple seed stain (PSS) of soybean caused by Cercospora kikuchii is an important disease that reduces market grade and can affect seed germination and vigor. A single dominant gene was shown to confer PSS resistance in PI 80837. The objective of this research was to map the PSS resistance gene in PI 80837 using simple sequence repeat (SSR) markers. A cross was made between the PSS-susceptible c...
متن کاملGenetic Transformation System for the Fungal Soybean Pathogen Cercospora kikuchii.
An altered beta-tubulin gene that confers resistance to the fungicide benomyl was isolated from a genomic library of a UV-induced mutant of Cercospora kikuchii and used as a selectable marker for transformation. The level of benomyl resistance conferred to the transformants was at least 150-fold greater than the intrinsic resistance of the C. kikuchii recipient protoplasts. In the majority of c...
متن کاملBcmfs1, a novel major facilitator superfamily transporter from Botrytis cinerea, provides tolerance towards the natural toxic compounds camptothecin and cercosporin and towards fungicides.
Bcmfs1, a novel major facilitator superfamily gene from Botrytis cinerea, was cloned, and replacement and overexpression mutants were constructed to study its function. Replacement mutants showed increased sensitivity to the natural toxic compounds camptothecin and cercosporin, produced by the plant Camptotheca acuminata and the plant pathogenic fungus Cercospora kikuchii, respectively. Overexp...
متن کامل