Properties of co conotoxin MVIIC receptors associated with 0 lA calcium channel subunits in rat brain
نویسندگان
چکیده
Solubilized ~2sI-¢o conotoxin MVIIC receptors from rat cerebellum were immunoprecipitated by antibodies directed against the calcium channel ~ I A subunit. Anti-alA antibodies recognized a 240-220, 180 and 160 kDa proteins in immunoblots of cerebellar membranes. Disuccinimidyl suberate cross-linked ~2sI-~o conotoxin MVIIC to an ct26-1ike 200-180 kDa subunit, which migrated at 150-140 kDa after disulfide reduction. These observations are consistent with a heteromeric structure in which high affinity to conotoxin MVIIC binding sites formed by a l A subunits are located in close proximity to peripheral ct2 subunits.
منابع مشابه
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