Amyloglucosidase from Rhizopus mold and β-glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides

Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and βglucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, Dmannose, D-ribose, sucrose, maltose and lactose, both the enzymes gave yields in the range of 5-40%. Amyloglucosidase catalysis gave C1α and C1β glycosides of D-glucose, D-galactose, D-mannose, D-ribose, maltose, C6-O-arylated products of D-glucose and maltose and C1-O-arylated product of sucrose. However, β-glucosidase gave C1β-glucoside, C1βlactoside and C1α and C1β glycosides of D-galactose and D-mannose. Riboflavinyl glycosides showed angiotensin converting enzyme inhibitory activities, with IC50 values in the 0.8±0.04-2.08±0.1 mM range.