Core promoter elements and TAFs contribute to the diversity of transcriptional activation in vertebrates.

Transcriptional Activation in Vertebrates Contribute to the Diversity of Core Promoter Elements and TAFs

Selective Recruitment of TAFs by Yeast Upstream Activating Sequences Implications for Eukaryotic Promoter Structure

The general transcription factor TFIID is composed of the TATA box binding protein (TBP) and multiple TBP-associated factors (TAFs). In yeast, promoters can be grouped into two classes based on the involvement of TAFs. TAF-dependent (TAF(dep)) promoters require TAFs for transcription, and TBP and TAFs are present at comparable levels on these promoters. TAF-independent (TAF(ind)) promoters do n...

Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II

The mechanisms that govern core promoter recognition and basal transcription efficiency remain poorly understood. Here, we have assessed the potential role of TAFs and the TFIID complex in directing basal promoter function. Reconstituted transcription reactions revealed the ability of TFIID versus TBP to discriminate between distinct core promoters. A comparison of different partial TBP-TAF ass...

Transcriptional Coactivator CBP Facilitates Transcription Initiation and Reinitiation of HTLV-I and Cyclin D2 Promoter

HTLV-I is the etiologic agent for adult T-cell leukemia/lymphoma (ATL) and HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Taxi, the major activator of this virus, is a 40- kDa (353 amino acid) phosphoprotein, predominantly localized in the nucleus of the host cell, which functions to trans-activate both viral and cellular promoters. Recently it has been shown that HTLV-I a...

Activation and repression mechanisms in yeast.

In eukaryotes, gene expression depends on activator proteins that bind enhancer elements and stimulate transcription by RNA polymerase II (pol II) (Struhl 1995; Zawel and Reinberg 1995). This general requirement for activators is inferred from numerous observations in vivo that intact promoters are much more efficiently transcribed than core promoter derivatives containing only the TATA and ini...