Isolation from a coastal fish of Vibrio hollisae capable of producing a hemolysin similar to the thermostable direct hemolysin of Vibrio parahaemolyticus.
نویسندگان
چکیده
A vibrio isolated from the intestine of a coastal fish was identified as Vibrio hollisae by its biochemical characteristics. The isolate reacted with the gene probe for the thermostable direct hemolysin of Vibrio parahaemolyticus. The hemolysin produced by the isolate from the fish had traits identical to those of the thermostable direct hemolysin-like hemolysin produced by a clinical strain of V. hollisae.
منابع مشابه
Characterization of a Vibrio alginolyticus strain, isolated from Alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus.
A Vibrio strain isolated from Alaskan oysters and classified by its biochemical characteristics as Vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in Vibrio parahaemolyticus. This trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of V. parahaemolyticus. This gene seems to be functional since it was ...
متن کاملOccurrence of scrub typhus (Tsutsugamushi) in Kanagawa Prefecture and types of Orientia tsutsugamushi involved.
gene (tdh) and the thermostable direct hemolysin-related hemolysingene (blh) of Vibrio parahaemolyticus by polymerase chain reaction. Mol. Cell. Probes, 6, 477-487. 3. Sugiyama, A., Nakano, Y., Iwade, Y., Yamauchi, A., Nakayama, 0., Matsumoto, T., Kou, Y., Ito, T., Nishinaka, T. and Shoji, T. (1 999): Isolation of thermostable direct hemolysin-producing Vibrio parahaemolyticusfrom food implicat...
متن کاملVibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques
Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. V. parahaemolyticus is the leading causal agent of human acute gastroenteritis following the consumption of raw, undercooked, or mishandled marine products. In rare cases, V. parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-exi...
متن کاملThe promoter region rather than its downstream inverted repeat sequence is responsible for low-level transcription of the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus.
We determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by using a PCR-based method and identified the promoter. Mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription.
متن کاملConstruction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)
A mutant, Vp-MX02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (TRH) was constructed by double-crossover gene conversion from a TRH-producing Vibrio parahaemolyticus strain, TH3996. The deleted region was the upstream half of trh. Hemolysis was completely lost, and TRH antigen was undetectable in the mutant. Administration of the mutant into ligat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 54 8 شماره
صفحات -
تاریخ انتشار 1988