Multiplex PCR assay for identification of six different Staphylococcus spp. and simultaneous detection of methicillin and mupirocin resistance.

نویسندگان

  • E Campos-Peña
  • E Martín-Nuñez
  • G Pulido-Reyes
  • J Martín-Padrón
  • E Caro-Carrillo
  • J Donate-Correa
  • I Lorenzo-Castrillejo
  • J Alcoba-Flórez
  • F Machín
  • S Méndez-Alvarez
چکیده

We describe a new, efficient, sensitive, and fast single-tube multiple-PCR protocol for the identification of the most clinically significant Staphylococcus spp. and the simultaneous detection of the methicillin and mupirocin resistance loci. The protocol identifies at the species level isolates belonging to S. aureus, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, and S. saprophyticus.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 52 7  شماره 

صفحات  -

تاریخ انتشار 2014