Method for rapid infrared microdetermination of serum carbonate.
نویسنده
چکیده
C ARBON DIOXIDE can be measured by infrared absorptiometry, so carbon dioxide evolved from a sample of serum or plasma by acid can be determined by this instrumental technique. Procedures published earlier based on this approach (1, 2) were not competitive with other techniques available for analysis of blood carbonates since they required 1.0 and 0.5 ml of blood, respectively, for the sample and approximately 3 mm for a determination. Both of these methods were relatively slow and insensitive because they depended upon evolution of the CO2 into a recirculating gas stream. With the method reported here, a determination can be made in 1 mm or less on a suggested 5O-il sample. Smaller samples can be used with some decrease in precision. The object of this method is to provide an additional capability in an automated laboratory for processing either very small samples or samples for which results are urgently needed separately from the automated analytical stream. The method is also useful for a laboratory which does not have a sufficiently large number of samples to justify an automated method. In contrast to the previous work, the CO2 evolved from the sample in acid solution is not recirculated but simply passes through a nondispersive gas analyzer. The absorption signal is integrated to provide a direct reading of the sample concentration on a digital voltmeter.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 16 2 شماره
صفحات -
تاریخ انتشار 1970