Studies on PTH-amino acids by high voltage filter paper electrophoresis.
نویسندگان
چکیده
منابع مشابه
Two-way separation of amino acids and other ninhydrin-reacting substances by high-voltage electrophoresis followed by paper chromatography.
One-way separation of amino acids by electrophoresis at high voltage is now a well-established procedure (Gross, 1955). With the large number of amino acids often found in biological fluids, it is, however, very difficult to obtain satisfactory separation without overlap of spots. Several investigators have used techniques for the separation of amino acids and other substances by a combination ...
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Two dimensional thin layer chromatography on polyamide sheets is a useful method for identification of PTH-amino acids which have very close Rf-values. The solvent systems described here are particularly effective and have an edge over other solvent systems used for the separation of PTH-amino acids. The solvent systems provide a clear separation of all PTH-amono acids except lle / Leu in a...
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Capillary zone electrophoresis provides a rapid method for the analysis of minute amounts of amino acids. A laser-based absorbance technique is used to determine phenylthiohydantoin amino acids with detection limits of 10.15 mot; separation of 20 amino acids, based on micelle modified zone electrophoresis, requires 13 minutes. The amino acid sequence of a protein is of fundamental value in the ...
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This method for assay of polyamines (putrescine, spermidine, and spermine) in large numbers of urine samples is based on preliminary analyte isolation by use of a Dowex 50W column, separation by high-voltage paper electrophoresis, reaction with ninhydrin to detect the separated amines, and subsequent direct assay with a dual-wave-length thin-layer chromatography scanner by the zigzag scanning m...
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A simple and accurate method for the determination of urinary 3-methoxy-4-hydroxy-mandelic acid, vanillyl-mandelic acid (VMA), is described. The separation of VMA from all other phenolic acids in approximately 0.05 ml. untreated urine is achieved by using low-voltage paper electrophoresis with different buffer concentrations for the cathode and the anode. The concentration of the electrophoreti...
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ورودعنوان ژورنال:
- Acta medica Nagasakiensia
دوره 12 1 شماره
صفحات -
تاریخ انتشار 1967