CHAPTER 15 The Polymerase Chain Reaction
نویسنده
چکیده
T he polymerase chain reaction (PCR) is a rapid procedure for in vitro enzymatic amplification of a specific segment of DNA. Like molecular cloning, PCR has spawned a multitude of experiments that were previously impossible. The number of applications of PCR seems infinite—and is still growing. They include direct cloning from genomic DNA or cDNA, in vitro mutagenesis and engineering of DNA, genetic fingerprinting of forensic samples, assays for the presence of infectious agents, prenatal diagnosis of genetic diseases, analysis of allelic sequence variations, analysis of RNA transcript structure, genomic footprinting, and direct nucleotide sequencing of genomic DNA and cDNA.
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