Development of high-throughput yeast-cell-based bioreporter assays for specific monitoring of bisphenol A and chemical testing of endocrine disrupting compounds
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چکیده
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منابع مشابه
Comparative potential of black tea leaves waste to granular activated carbon in adsorption of endocrine disrupting compounds from aqueous solution
The adsorption properties and mechanics of selected endocrine disrupting compounds; 17 β-estradiol, 17 α – ethinylestradiol and bisphenol A on locally available black tea leaves waste and granular activated carbon were investigated. The results obtained indicated that the kinetics of adsorption were pH, adsorbent dose, contact time and temperature dependent with equilibrium being reached at 20 ...
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Saccharomyces cerevisiae bioluminescent bioreporter assays were developed previously to assess a chemical's estrogenic or androgenic disrupting potential. S. cerevisiae BLYES, S. cerevisiae BLYAS, S. cerevisiae BLYR, were used to assess their reproducibility and utility in screening 68, 69, and 71 chemicals for estrogenic, androgenic, and toxic effects, respectively. EC(50) values were 6.3 +/- ...
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Many of the > 83,000 chemicals currently used in commerce pose potential toxic hazards to human health and the environment. Numerous policies and systems are in place to try to identify harmful chemicals that are already in use, but removing these problem chemicals once they are already in the marketplace can be difficult, time-consuming, and costly. In an ideal world, chemists would be able to...
متن کاملUse of Saccharomyces cerevisiae BLYES expressing bacterial bioluminescence for rapid, sensitive detection of estrogenic compounds.
An estrogen-inducible bacterial lux-based bioluminescent reporter was developed in Saccharomyces cerevisiae for applications in chemical sensing and environmental assessment of estrogen disruptor activity. The strain, designated S. cerevisiae BLYES, was constructed by inserting tandem estrogen response elements between divergent yeast promoters GPD and ADH1 on pUTK401 (formerly pUA12B7) that co...
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Aquatic environments receive significant levels of chemical contaminants generated by human activities. Among these pollutants, we noticed the xenobiotics known as reproductive toxicants and endocrine disruptors. The endocrine disruption in wildlife has been the subject of many reviews and workshops in recent years. Field observations of reproductively abnormal organisms and population declines...
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