An immunoblotting procedure comprising O = 9,12 and H = d antigens as an alternative to the Widal agglutination assay.
نویسندگان
چکیده
AIMS To compare the established Widal agglutination assay with an immunoblotting procedure. METHODS 110 sera were used to compare the established Widal agglutination assay with an immunoblotting procedure incorporating lipopolysaccharide (LPS) (O = 9,12) and flagellar (H = d) antigens. RESULTS Antibodies to the LPS antigens were detected in 18 sera by the Widal assay and in 37 by immunoblotting. Antibodies to the flagellar antigens were detected in 27 sera by Widal assay and in 25 by immunoblotting. CONCLUSIONS An immunoblotting procedure incorporating O = 9,12 LPS and H = d flagellar antigens was rapid and more sensitive than the established Widal agglutination assay for providing evidence of infection with S typhi.
منابع مشابه
The serodiagnosis of infection with Salmonella typhi.
BACKGROUND/AIMS The serodiagnosis of infection with Salmonella typhi, using the Widal agglutination assay, relies on patients' antibodies to the O = 9,12 lipopolysaccharide (LPS) antigens, H = d flagellar antigens, and the Vi capsular antigens. A Vi agglutination titre of > 1/40 has traditionally been regarded as indicative of recent infection with S typhi. In this study, 91 sera were used to a...
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Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. This study was performed to assess the effect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 +/- 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among ...
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A passive haemagglutination test, using sheep red blood cells sensitised with Salmonella typhi lipopolysaccharide, was compared with the Widal test for the serological diagnosis of typhoid fever in an endemic area. The results obtained on sera from 152 patients with bacteriologically confirmed typhoid and 183 patients who did not have typhoid were analysed in terms of sensitivity, specificity, ...
متن کاملModifications In!uencing Widal Test Reactivity in a Novel Microplate Assay
Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. !is study was performed to assess the e$ect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 + 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among them...
متن کاملSerological response of patients infected with Salmonella typhi.
AIMS To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. METHODS A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used. RESULTS Seventy two of 73 sera contained antibod...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 51 11 شماره
صفحات -
تاریخ انتشار 1998