A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells.
نویسندگان
چکیده
We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. The probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. The fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and RAW264.7 cells intracellular free extracts. We also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. Confocal microscopy experiments using mouse macrophage cell line RAW264.7 show that PyBor is able to detect the different intracellular peroxynitrite levels. In addition, we have performed quantum chemical calculations with TD-DFT/M06/TZVP level with COSMO solvation model basis sets using a suite of Gaussian 09 programs to provide insights into the structure optical properties of PyBor and PyOH.
منابع مشابه
A water-soluble boronate-based fluorescent probe for the selective detection of peroxynitrite and imaging in living cells†
(2014) A water-soluble boronate-based fluorescent probe for the selective detection of peroxynitrite and imaging in living cells. This version is made available in accordance with publisher policies. Please cite only the published version using the reference above. A water-soluble boronate-based fl uorescent probe for the selective detection of peroxynitrite and imaging in living cells A water-...
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ورودعنوان ژورنال:
- The Analyst
دوره 137 16 شماره
صفحات -
تاریخ انتشار 2012