The Effects of Purified Recombinant Murine tnterleukin-3 and/or Purified Natural Murine CSF-1 In Vivo on the Proliferation of Murine High- and Low-Proliferative Potential Colony-Forming Cells: Demonstration of In Vivo Synergism
نویسندگان
چکیده
Purified natural murine I cell (macrophage) colony-stimulating factor (nCSF-1 ) and purified recombinant murine interleukin-3 (rlL-3) were administered to normal or lactoferrin-pretreated mice 20 to 24 hours before sacrifice. rlL-3 and nCSF-1 administered separately increased the percentage of macrophage high-proliferative potential colonyforming cells (HPP-CFC) and low-proliferative potential colony-forming cells (LPP-CFC) in active cell cycle. Endotoxin was not detected in the samples of nCSF-1 or rIL-3 with the Limulus lysate test. and the in vitro and in vivo hematopoietic stimulatory effects of both molecules were
منابع مشابه
Proliferate Resonse to Purified Bordetella pertussis Toxin on Murine Spleen Lymphocytes
A purification procedure of pertussis toxin (PT) from submerged culture of Bordetella pertussis (B.pertussis) strain 134 using adsorption and affinity chromatography was discussed. The yield of the resulting PT was approximately 37.5mg/l of concentrated culture supernatant. The polypeptide pattern of the purified PT was investigated by SDS-PAGE and showed five bands between 11 to 26 KDa using l...
متن کاملDIFFERENTIAL EXPRESSION OF SURFACE MARKERS CD45RB AND CD44 ON MURINE CD8+ CELLS
Considering the emerging importance of phenotypic markers as indicators of cell function and differentiation, we studied patterns ofCD44 and CD45RB expression in CD8+ murine T cells with prior exposure to antigen or staphylococcal enterotoxin B ( SEB ). Following in vivo priming with two purified protein derivatives (one from a virulent WHO strain and the other from an avirulent strain), T ...
متن کاملIN VITRO STUDY OF AN ENDOGENOUS IMMUNOSUPPRESSOR FACTOR DERIVED FROM HUMAN OR BOVINE SERUM
The effects of the human and bovine LSF (derived from sera) as well as their purified fractions were studied on murine lymphocytes reactions indicated by blast transformation (BT) assay, mixed lymphocyte culture (MLC) and IgG synthesis. The results indicated that bovine lipid suppressor factor (LSF) has significant immunosuppressive activity on lymphocytes proliferation both in BT and MLC ...
متن کاملInterleukin-1 alpha enhances the in vitro survival of purified murine granulocyte-macrophage progenitor cells in the absence of colony-stimulating factors.
Interleukin-1 (IL-1), recently identified as hemopoietin-1, affects hematopoiesis by presumably direct and indirect mechanisms. IL-1 stimulates primitive hematopoietic stem cells to express receptors for the granulocyte-macrophage colony-stimulating factors (GM-CSFs) and stimulates CSF release from accessory cells. We used highly purified murine granulocyte-macrophage progenitor cells (CFU-GM, ...
متن کاملColony-stimulating Factor-1, and Recombinant Murine Granulocyte-Macrophage Colony-stimulating Factor on Myelopoiesis in Mice
Purified murine colony-stimulating factors (CSF) recombinant interleukin 3 (IL-3), natural CSF-1, and recombinant granulocyte-macrophage (GM) CSF were assessed in vivo for their effects on BDF, mouse bone marrow and spleen granulocyte-macrophage (CFU-GM), erythroid (BFU-E), and multipotential (CFU-GEMM) progenitor cells in untreated mice and in mice pretreated with purified iron-saturated human...
متن کامل