The use of a whole-blood benchtop analyzer (Nova 16) in a cardiac STAT intensive care unit.

␮L of 0.5 mg/L peroxidase-linked RCA 120 in BSA/PB. Because this lectin can bind to immobilized immuno-globulins through their carbohydrate moiety, we ran a blank test in which no serum was added; the absorbances of these blanks, always Ͻ0.15, were acceptable. Concentrations of the dSHBG standard solutions ranged from 0 to 500 ␮g/L (Fig. 1B). Serum samples were usually diluted 1:286 and 1:546 in BSA/PB; however, for some patients, higher dilutions had to be tested. All determina-tions were done in duplicate, and data were analyzed as previously described for total SHBG. The within-run CV for duplicates was ϳ7% (n ϭ 173), and mean analytical recovery (overload ϭ 30 ␮g/L) was 105% (SD ϭ 17%, n ϭ 40). Because the in vivo desialyla-tion process may affect the glycan chains differently, serum probably contains several SHBG isoforms with various sialic acid or terminal galactosyl residues contents and thus with different reactivities toward RCA 120. Because of this heterogeneity in the serum isoform profile, the use of in vitro neuraminidase-treated SHBG as a standard is questionable. Serum dSHBG concentrations were thus expressed as arbitrary units, one unit being defined as the absorbance obtained with a 1 ␮g/L dSHBG calibrator. In healthy donors, values range from 9 to 12 U/L, whereas in some patients with hepatic diseases, concentrations may rise to 100 U/L. SHBG and dSHBG can be determined in parallel easily using the described procedures because most of the steps are similar: coating with anti-SHBG antibody, BSA saturation , conditions for washing and incubation, enzymatic amplification, photometric measurement, and data analysis. Because total SHBG determination can be measured in a low ionic strength buffer, i.e. PB, the two assays differ only by (a) the nature of the peroxidase-conjugate (anti-SHBG for total SHBG and RCA 120 for dSHBG), (b) the serum dilution, and (c) the standard for calibration. The convenience of this coupled procedure allows extensive studies of various pathological conditions, such as hepa-tocellular carcinoma, and may thus be useful for a better knowledge of the physiopathology of this disease. deglycosylation of native sex steroid-binding protein (SBP or SHBG) of human and rabbit plasma: effect on the steroid-binding activity. Predictive value of serum sex hormone binding globulin for the occurrence of hepatocellular carcinoma in male patients with cirrhosis. A liquid phase immunoradiometric assay (IRMA) for human sex hormone binding globulin (SHBG). A. Two-site immunoradio-metric assay using monoclonal antibodies for the determination of serum human …