Mapping fertility-restoring genes of rice WA cytoplasmic male sterility using SSLP markers
نویسندگان
چکیده
Rice wild abortive (WA) type cytoplasmic male sterility (CMS) is commercially used for production of hybrid seeds in China. Two fertility restorer genes in the CMS-WA system were detected with SSLP markers in this study. An F 2 population consisting of 210 excessive sterile individuals from a cross between Zhenshan 97A and a strong restorer line IR24 was used for mapping of Rf4. The genetic distance from Rf4 locus to RM171 (OSR33) and RM228 on long arm of chromosome 10 was 3.7 cM and 3.4 cM, respectively, which were the two closest SSLP markers flanking the Rf4 locus. The two SSLP markers gave promise of application in molecular marker-assisted selection (MAS) for fertility restorer lines of the CMS-WA system. RM244, another SSLP marker on the short arm of chromosome 10, was found to be linked with a fertility restorer locus in an F 2 population consisting of 30 excessive sterile individuals from a cross between Zhenshan 97A and a weak restorer line IR64. The genetic relationship among fertility restorer genes Rf1, Rf4 and Rf5(t) for three rice CMS system is also discussed.
منابع مشابه
Inheritance of the fertility restoration and genotyping of rice lines at the restoring fertility (Rf) loci using molecular markers
The combination of cytoplasmic male sterility (CMS) in one parent and a restorer gene (Rf) to restore fertility in another are indispensable for the development of hybrid varieties. To genotype rice lines at the restoring fertility (Rf) loci, 38 lines were crossed with a sterile tester (rfrf) line. Pollen fertility test was performed to identify sterile and fertile F1 hybrids. Seven lines were ...
متن کاملThe Possible Role of Atp6 Gene in Cytoplasmic Male Sterility in WA (Wild Abortive) Type of Rice (Oryza sativa L.)
The possibility of occurrence of some rearrangements inside and or around coxII and atp6 genes and their relationship with male sterility in rice lines having wild abortive (WA) cytoplasm was studied. Two sterile (IR58025A and IR62829A) and two maintainer lines (IR58025B and IR62829B) were used. Radioactive Southern blotting was employed to carry out the experiments. The hybridization of coxII...
متن کاملINHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)
Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated t...
متن کاملEdects ofRit 1 , Rit 3 and Rif 6 ( D Genes on Fertility Restoration in Rice ( 0 i : yza sativa L . ) with WA - and BT - type Cytoplasmic Male Sterility
Genes involved in the fenility restoration (llf) ofwild-abortive (WA)-type cytoplasmic male sterility (CMS) in rice have not been clearly identified, because of the inconsistency of their number and map position, Although the IILI genes, which restore Chinsurah Boro (BT)-type CMS, have been recently isolated, their efi fects on WA-type CMS are still unclear, In the present study, near-isogenic ...
متن کاملMapping of rice Rf gene by bulked line analysis.
A method, bulked line analysis (BLA), was developed for identification of the RFLP markers associated with a target gene. Instead of segregating progenies, conventional lines sharing the same trait were bulked by the BLA method. This method is an alternative approach to the identification of DNA markers linked with a target gene. A major advantage of this method is time-saving for genetic stock...
متن کامل