Quantitative real-time RT-PCR based transcriptomics: Improvement of evaluation methods
نویسنده
چکیده
The stability of standard gene expression is an elementary prerequisite for internal standardisation of target geneexpression data and many so called housekeeping genes with assumed stable expression can exhibit either up-or down-regulation under some experimental conditions. The developed, and herein presented, software calledBestKeeper determines the best suited standards, out of ten candidates, and combines them into an index. Theindex can be compared with further ten target genes to decide, whether they are differentially expressed under anapplied treatment. All data processing is based on crossing points. The BestKeeper software tool was validatedon four housekeeping genes and 10 members of the somatotropic axis differentially expressed in bovine corporalutea total RNA. The BestKeeper application and necessary information about data processing and handling can bedownloaded on http://www.wzw.tum.de/gene-quantification/bestkeeper.html
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