Quantitative gas-liquid chromatographic analysis of ethanolamine, monomethyl ethanolamine, and dimethyl ethanolamine from lipids.

نویسندگان

  • R L Lester
  • D C White
چکیده

A method for the separation and quantitative estimation of ethanolamine, dimethyl ethanolamine, and monomethyl ethanolamine derived from lipids is described. After acid hydrolysis of the lipids, the bases are extracted from the neutralized hydrolysate with t-butonol and separated by gas-liquid chromatography.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

31P NMR of phospholipid glycerol phosphodiester residues.

Saponification of extracted tissue phospholipids yields a set of isolated glycerol 3-phosphoryl phospholipid polar headgroups from which semi-quantitative 31P NMR spectra can be obtained. The resonance signals from these molecules, which frequently have been reported as uncharacterized phosphate signals observed in perchloric acid extracts of tissue, can be used as an aid in the characterizatio...

متن کامل

Base- and acid-catalyzed interconversions of O-acyl- and N-acyl-ethanolamines: a cautionary note for lipid analyses.

The isolation and quantification of ethanolamine containing lipids from animal tissues may expose neutral lipid extracts to acidic or basic conditions during chromatographic separations or derivatization chemistry. While investigating the acid- and base-catalyzed production of anandamide in chromatographic fractions of rat brain extracts not containing anandamide, we observed that O,N-acyl migr...

متن کامل

Di-ethanolamine Might Cause Bone-related Complications Due to the Reduction of Osteogenic Differentiation and Induction of Oxidative Stress

Di-ethanolamine (DEA) is a well-known environmental pollutant used in manufacturing soap, detergent, body lotion, and other sanitary products. DEA has been reported to cause cytotoxicity in different tissue and cell, but no study was found to explain the toxic effect of DEA on rat bone marrow mesenchymal stem cells (BMSCs) differentiation. Thus in the present study, the differentiation property...

متن کامل

Inhibition of Copper Corrosion by Ethanolamine in 100 ppm NaCl

We study copper corrosion inhibition by ethanolamine (ETA)with (0, 0.2 ,0.3,0.4,0.5) vol.% concentrations in 100 ppm NaCl solution. This work is carried out by potentiodynamic measurements and Electrochemical Impedance Spectroscopy (EIS). The substrates' surface morphologies are examined by Scanning Electron Microscopy (...

متن کامل

The mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. The source of the third methyl hydrogen in the 5'-deoxyadenosine generated from the cofactor during catalysis.

Ethanolamine ammonia-lyase is an adenosylcobalamin-dependent enzyme which catalyzes the conversion of ethanolamine and propanolamine to ammonia and the corresponding aldehydes. A mechanism has been proposed for this and other adenosylcobalamin-dependent reactions which involves cleavage of the carbon-cobalt bond of the cofactor followed by abstraction of a substrate hydrogen atom by the adenosy...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of lipid research

دوره 8 6  شماره 

صفحات  -

تاریخ انتشار 1967