Semiautomated method for analysis of 11-deoxy-17-oxo steroids in urine. A preliminary report.
نویسنده
چکیده
A method is described for the automated analysis of urinary 11-deoxy-17-oxo steroids from hydrolyzed conjugate extracts. Steroid conjugates are extracted manually on an Amberlite XAD-2 column and hydrolyzed with f3-glucuronidase at 62#{176}C for 90 mm. After addition of alkali, the samples are transferred to the AutoAnalyzer where the steroids are extracted into isooctane and estimated colorimetrically by a modified Zimmerman reaction.
منابع مشابه
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 19 7 شماره
صفحات -
تاریخ انتشار 1973