Interleukin - 3 and Interleukin - la Allow Earlier Bone Marrow Progenitors To Respond to Human Colony - Stimulating
نویسندگان
چکیده
By using human bone marrow cells enriched for early progenitors by selective immunoadsorption and plated at low cell density (iO to iO cells/mL/9.6 cm2) in semisolid methylcellulose culture. we have analyzed the cooperative effects of human colony-stimulating factor 1 (CSF-1 ), granulocyte-macrophage-CSF (GM-CSF). interleukin-1 a (ILla). and gibbon as well as human recombinant IL-3 on the formation of monocytic colonies. CSF-1 alone stimulated mature monocytic colony formation by human CFU-M. However. in the presence of IL-3 and erythropoietin. CSF-1
منابع مشابه
Interleukin-3 and interleukin-1 alpha allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1.
By using human bone marrow cells enriched for early progenitors by selective immunoadsorption and plated at low cell density (10(3) to 10(4) cells/mL/9.6 cm2) in semisolid methylcellulose culture, we have analyzed the cooperative effects of human colony-stimulating factor 1 (CSF-1), granulocyte-macrophage-CSF (GM-CSF), interleukin-1 alpha (IL-1 alpha), and gibbon as well as human recombinant IL...
متن کاملProliferative properties of unfractionated, purified, and single cell human progenitor populations stimulated by recombinant human interleukin-3.
In this report, the biological properties of human recombinant interleukin-3 (rhIL-3) were studied. We investigated the range of unfractionated, purified and single cell human progenitors responsive to IL-3; compared the colony types observed with those obtained in the presence of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or granulocyte-CSF (G-CSF). The results...
متن کاملRegulation of Human Eosinophil Precursor Production by Cytokines: A Comparison
The effect of a panel of recombinant human (rh) cytokines on the generation of human eosinophil precursors was assessed using a two-step culture technique. Normal human bone marrow was preincubated with different cytokine combinations in liquid culture before assessment of the number of eosinophil progenitors, which give rise to eosinophil colony-forming units (CFU-Eo) on secondary semi-solid c...
متن کاملKidney cell lysates contain an activity that stimulates mature erythroid burst-forming-unit (mBFU-E) proliferation.
This study reports the detection of an activity that stimulates the development of a subclass of burst-forming unit-erythroid (BFU-E) progenitors giving rise to small bursts in semi-solid cultures established in the presence of saturating concentrations of erythropoietin. These progenitors are considered to be mature BFU-E. The activity is found in extracts from kidney cells and appears to be p...
متن کاملRegulation of human monocyte DNA synthesis by colony-stimulating factors, cytokines, and cyclic adenosine monophosphate.
It is reported in this study that a subpopulation of highly purified human peripheral blood human monocytes can proliferate in response to colony-stimulating factor-1 (CSF-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3). Both GM-CSF and IL-3 synergized with CSF-1 for the induction of DNA synthesis. Given the DNA synthesis levels attained, we were able to ...
متن کامل