A Microfluidic Model to Study the Metastatic Cascade: from Adhesion to Migration

نویسندگان

  • W. A. Velema
  • P.P.M.F.A. Mulder
  • L. P. Lee
  • E. Verpoorte
چکیده

This paper presents a microfluidic model for studying the sequential steps of the cancer extravasation process, in which cancer cells exit capillaries and enter organs. We demonstrate the functionality of this model by co-culturing and observing the adhesion of SK-Mel-28 (Malignant Melanoma) cells to a Human Umbilical Vein Endothelial Cell (HUVEC) monolayer, followed by their migration through this HUVEC layer into gelatin-filled microgaps.

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تاریخ انتشار 2010