Routine ultramicro-measurement of human growth hormone in plasma by solid-phase radioimmunoassay.

plasma based on the solid-phase radioimmunoassay (RIA) using antibody-coated disposable microtiter trays was described. This method is simple, rapid and inexpensive,and particularly useful for ultramicro-measurement of plasma HGH levels in large numbers of plasma samples. The lower limit of sensitivity for purified HGH was 25pg/ml (P < 0.05). The precision of the measurement of HGH in plasma has been calculated, and the quantitative sensitivity of the method derived from the results given by its routine use was 56.8pg/ml (P<0.05). The HGH level of normal pooled plasma in 10 replicates was 1,515pg/ml