Analyzing yeast colonies by PCR.

MATERIALS Taq DNA polymerase Marker DNA As a reference, use single-stranded DNA from the original bacteriophage M13 recombinant. MgCl2 (25 mM) Oligonucleotide primers The oligonucleotides should be 20-24 nucleotides in length, specific for the target DNA sequences, free of potential secondary structures, and contain no less than 10 and no more than 15 G and C residues. Yeast colony PCR buffer, 10x Yeast strain carrying recombinant YAC of interest dNTP solution (10 mM) containing all four dNTPs (pH 8.0; PCR grade)