Supercritical Fluid Polarity-Based Fractionation of Plant Products
نویسندگان
چکیده
Fractionation of plant compounds to produce active purified fractions is a multi-step process, time-consuming and sometimes very expensive. This paper presents a single-step fractionation of tannins from a local plant, Phyllanthus niruri Linn. using supercritical fluid extraction (SFE) method. The effects of operating conditions (pressure, temperature, flow rate, cosolvent content and ethanol-water content in cosolvent) were investigated and these parameters were optimized by a response surface method using a face-centred central composite design for maximum yields. SFE produced fractionation between the less polar compounds (pre-extraction/pretreatment) and the more polar tannins (main extraction). At optimum conditions, higher contents could be obtained compared to a commercial P. niruri product, HEPAR-P, in terms of gallic acid, corilagin, ellagic acid, and total flavonoids. Beside reducing the total liquid solvent consumption and fractionation time, the SFE method developed could enhance both the pre-extraction and main extraction yields, and produce fractionation of different polarity compound groups from the plant matrices.
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