Localization of the 'apical body' in guinea-pig and human spermatozoa with phosphotungstic acid.
نویسنده
چکیده
acrosome vesiculate, releasing the acrosomal contents (Austin & Bishop, 1958; Austin, 1963; Hadek, 1963; Piko & Tyler, 1964; Barros, Bedford, Franklin & Austin, 1967; Bedford, 1967b). The inner acrosome membrane then becomes the outer ensheathment of the sperm head. Under these circumstances, the contents of the compartment between the inner acrosome membrane and the nucleus may be important for the movement of the spermatozoon through the zona and fusion of spermatozoon and ovum. Since it resides in the subacrosomal space at the apex of the nucleus, the 'apical body' is in a strategic locus for the approach of the spermatozoon toward the egg. The apical body, however, is a controversial structure since it has been reported in some mammals (Hadek, 1963; Nicander & Bane, 1966; Bedford, 1967a) but it has not been seen in others (Fawcett & Ito, 1965). Among the primates, Bedford (1967a) noted a subacrosomal body in the bush baby but it was not present in man. Because of the enormous development of the guinea-pig acrosome (Fawcett, 1965), this species is particularly favourable for analysis of the organelle and related substructures. An apical body, however, has not been consistently observed in the guinea-pig. It was described as a diffuse mass by Nicander & Bane (1966) but it was not present in an extensive study by Fawcett (1965). It was decided in the present study to reinvestigate the apical body in guineapig spermatozoa and spermatids treated en masse with ethanolic phosphotungstic acid (E-PTA). This technique (Gordon & Bensch, 1968) was chosen for several
منابع مشابه
Morphometrical Study of the Temporal Bone and Auditory Ossicles in Guinea Pig
In this research, anatomical descriptions of the structure of the temporal bone and auditory ossicles have been performed based on dissection of ten guinea pigs. The results showed that, in guinea pig temporal bone was similar to other animals and had three parts; squamous, tympanic and petrous .The tympanic part was much better developed and consisted of oval shaped tympanic bulla with many re...
متن کاملRegulation of proacrosin conversion in isolated guinea pig sperm acrosomal apical segments.
Following sodium dodecyl sulfate-polyacrylamide gel electrophoresis, proacrosin has been identified in extracts of intact guinea spermatozoa as a major silver staining band which reacted immunologically with antibodies made against purified proacrosin from guinea pig testis. Proacrosin exhibited an approximate Mr of 50,000 and was rapidly converted to an Mr 45,000 protein following induction of...
متن کاملKaryotype of Hairless Guinea pig
Chromosomal patterns of experimental animals are useful tools for cytogenetics research and animal breeding. Chromosome investigations of the hairless guinea pig are rare, therefore, karyotype of hairless guinea pigs (twelve male and female) was studied using metaphase spreads of bone marrows and G banding techniques. The chromosomes diploid number was 2n= 64 and polymorphism of three type chro...
متن کاملC-Kit expression in the gallbladder of guinea pig with chronic calculous cholecystitis and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal
Objective(s): To study the c-Kit expression in the gallbladder of cholesterol lithogenic guinea pig model and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal (ICCs). Materials and Methods:A total of 45 guinea pigs were randomly assigned into three groups: the control group (guinea pigs fed a standard diet, normal group); the model group (guinea pigs fed a cholesterol gal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of reproduction and fertility
دوره 19 2 شماره
صفحات -
تاریخ انتشار 1969