Kinetic nephelometric procedure for measurement of amylase activity in serum.
نویسندگان
چکیده
We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the new procedure is 3.4% (relative standard deviation). Units of amylase activity can either be expressed in terms of milligrams of starch consumed or converted to more conventional amylase units.
منابع مشابه
Amylase activity in serum and urine: comparison of results by the amyloclastic, dyed-starch, and nephelometric techniques.
We assayed serum and urine specimens for amylase activity by the nephelometric (I),dyed-starch (Amylochrome) (II), and mayloclastic (III) techniques. For serum, the correlation coefficients of the regression lines were: I vs. II, 0.978 (n = 106); I vs. III, 0.736 (n = 110); and II vs. III, 0.739 (n = 108). For urine, they were I vs. II, 0.938 (n = 49); and I vs. III, 0.752 (n = 46). Because cal...
متن کاملNew saccharogenic determination of alpha-amylase in serum and urine.
I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed befo...
متن کاملInfluence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme
In this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (ZnFe2O4@SiO2-NH2) is reported. For doing so, at first, the magnetic nanoparticles of ZnFe2O4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy sil...
متن کاملRapid quantitative, specific measurement of pancreatic amylase in serum with use of a monoclonal antibody.
In this rapid quantitative assay for pancreatic alpha-amylase (EC 3.2.1.1) in serum, we precipitate salivary amylases by 10-min incubation with monoclonal anti-salivary amylase antibody immobilized on particles of polyvinylidene fluoride. We then centrifuge the serum mixture and measure the pancreatic amylase activity remaining in the supernate by a kinetic method. The assay requires 50 microL ...
متن کاملRapid estimation of amylase activity of serum by turbidimetry.
HE CHOICE OF RAPID METHODS for estimation of serum amylase activity is limited (1). Such methods depend upon the change in color of starch with iodine brought about by splitting of the starch by the enzyme. it is often difficult to establish an end point visually by this reaction in partially digested starch solutions, especially by those whose experience with the method is limited. Furthermore...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 18 11 شماره
صفحات -
تاریخ انتشار 1972