Study of Hydrophobicity and Prediction of Antigenic Epitope of NADH dehydrogenase subunit 5 from D. medinensis

In this study NADH dehydrogenase subunit 5 (mitochondrion) protein has been used to investigate its role in antigenicity. NADH dehydrogenase subunit 5 (mitochondrion) protein is an active protein in mitochondria and it is a part of a large enzyme complex known as complex I. Complex I play an important role in oxidative phosphorylation. The NADH dehydrogenase complex (or mitochondrial respiratory complex I) catalyzes the oxidation of NADH by ubiquinone. This reaction is linked to proton transfer across mitochondrial membranes [1]. Mutation in the mitochondrial NADH dehydrogenase subunit 5(MT-ND5) causes mitochondrial encephalomyopathy, lactic acidosis, and strokelike episodes (MELAS), Leigh’s syndrome and Leber’s hereditary optic neuropathy. It has been found that mitochondrial ND5 12338T>C variant is generally associated with maternally inherited hypertrophic cardiomyopathy in a Chinese pedigree and this investigation suggest that thetheoretical basis for genetic counseling is required for maternally inherited hypertrophic cardiomyopathy [2]. The mutation of the mitochondrial ND5 T12338C is found to be associated with Leber’s hereditary optic neuropathy which has been investigated in the two chinese families [3]. Investigation reveals that the mitochondrial ND5 as the causative gene of Leight syndrome [4]. Guinea worm is the largest tissue parasite which is the only species that infect humans. It has got very unusual life cycle with incubation period of the approximately more than a year with six developmental stages. This one of the most neglected tropic parasite which bears clinical importance and needs to be eradicated after small pox [5]. Mature and adult female after the copulation produces millions of eggs in its uterus, and is predominantly localized in the lower extremities (80-90%). After an incubation period the female worm release the larvae which induces a painful blister (1 to 6cm diameter ) on the skin of lower limbs; the person developa slight fever , localskinredness , swelling and severe pruritus around the blister . Other symptoms include diarrhea, nausea, vomiting and dizziness. The blister burst within 1 to 3 days and female worms one or more slowly comes out from the wounds which causes an excoriating burning sensation *Corresponding author: Virendra S. Gomase, Department of Biotechnology, Mewa r University, Chittorgarh, India, Tel: 01471 220 881; E-mail: [email protected]