Partially Purification and Characterization of Polyphenol Oxidase of Quince
نویسندگان
چکیده
In fruits and vegetables, browning occurs due to the oxidation of polyphenols. Oxidative browning is catalysed by polyphenol oxidase (PPO; monophenol, dihydroxyphenyl alanine:oxygen oxidoreductase; EC.1.14.18.1). When fresh products are damaged oxidative browning occurs, and this is an economic problem for producers and consumers. Polyphenoloxidase, which is a copper-containing enzyme, catalyses the aerobic regioselective oxidation of monophenols to o-diphenols followed by dehydrogenation to o-quinons. PPO is widely distributed in plants and microorganisms and many researchers are interested in the PPO isolated from various sources such as the banana, Amasya apple, kiwi fruit, edible burdock, head lettuce , pear, palmito, cocoa bean and oil bean. However, there are only a few articles about quince PPO . The purpose of this study was to isolate PPO from quince and determine some of its enzymatic properties. In the study, the characteristics of the enzyme were investigated at different pH levels and temperatures. Substrate and inhibitor effects were also studied. The assays of PPO activity were carried out in the presence of air oxygen.
منابع مشابه
Partial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
متن کاملPartial purification and characterization of cresolase and catecholase activity of black mulberrys (Morus nigra)
Polyphenol oxidase from Black mulberrys was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. P-cresol was the better substrate for cresolase activity with a Km (11.5 mM) and catechol for catecholase activity with Km (6.4 mM) at pH 5. Km for cresolase and catecholase activity of IsoPPOII at pH 6.5 were 12 and 8.5 and for IsoPPOIII at pH ...
متن کاملThe Enzymatic Decolorization of Textile Dyes by the Immobilized Polyphenol Oxidase from Quince Leaves
Water pollution due to release of industrial wastewater has already become a serious problem in almost every industry using dyes to color its products. In this work, polyphenol oxidase enzyme from quince (Cydonia Oblonga) leaves immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extra...
متن کامل