Cytochemical Studies on the Chicken Erythrocyte
نویسنده
چکیده
Chicken erythrozytes possess many characteristics which make them a desirable material for experimental studies in cytochemistry, particularly those dealing with the chemical composition of various structural components within the cell. Avian erythrocytes have been used as a convenient source of material for the isolation of nuclei. Investigations along such lines were reported as early as 1869 by Plosz (1) and Ackermann (2), in 1904, prepared ntxlei from chicken erythrocytes by laking cells in distilled water and precipitating the nuclei in 3.G per cent sodium chloride. Warburg (3) used freezing and thawing as a technique for isolating nuclei. More recently, however, Laskowski (4) recommended lysolecithin as a hemolyzing agent and Dounce and Lan (5) suggested saponin for this purpose. Isolated chicken erythrocyte nuclei have been used as a source of material for recent biochemical studies on the composition of chromosomes (6)) electron microscope studies of isolated chromosomes (7)) and studies on the influence of irradiation on the respiration of nuclei (8). This report presents data on the distribution of certain amino acids in the proteins of the nucleus and cytoplasm of the chicken erythrocyte. Data are also given on the dry weight and nitrogen content of whole cells and isolated nuclei.
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