Journeying down the long and winding road: the whole picture of volume-activated Cl- channel activation in cardiac myocytes.
نویسندگان
چکیده
منابع مشابه
Chloride conductance is activated by membrane distention of cultured chick heart cells.
OBJECTIVE The aim was to apply various maneuvers to perturb the volume of cultured chick cardiac myocytes and to evaluate the association between the swelling-activated chloride conductance and membrane distention. METHODS Swelling of single chick heart cells was induced by (1) reduction of external osmolarity; (2) elevation of intracellular osmolarity; (3) isosmotic urea uptake; and (4) posi...
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The present study was designed to observe the properties of swelling-activated chloride channel (ICl.swell) in mouse cardiac myocytes using patch clamp techniques. In whole-cell recordings, hypotonic solution activated a chloride current that exhibited outward rectification, weak voltage-dependent inactivation, and anion selectivity with permeability sequence of I- > Br- > Cl-. The current was ...
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The role of protein kinase C (PKC) in regulating the protein kinase A (PKA)-activated Cl- current conducted by the cardiac isoform of the cystic fibrosis transmembrane conductance regulator (cCFTR) was studied in guinea pig ventricular myocytes using the whole cell patch-clamp technique. Although stimulation of endogenous PKC with phorbol 12,13-dibutyrate (PDBu) alone did not activate this Cl- ...
متن کاملSwelling-activated and isoprenaline-activated chloride currents in guinea pig cardiac myocytes have distinct electrophysiology and pharmacology
We have used the whole-cell patch clamp recording technique to characterize a swelling-activated chloride current in guinea pig atrial and ventricular myocytes and to compare the electrophysiological and pharmacological properties of this current with the isoprenaline-activated chloride current in the same cell types. Osmotic swelling of guinea pig cardiac myocytes caused activation of an outwa...
متن کاملF-actin modulates swelling-activated chloride current in cultured chick cardiac myocytes.
The integrity of F-actin and its association with the activation of a Cl- current ( I Cl) in cultured chick cardiac myocytes subjected to hyposmotic challenge were monitored by whole cell patch clamp and fluorescence confocal microscopy. Disruption of F-actin by 25 μM cytochalasin B augmented hyposmotic cell swelling by 51% (from a relative volume of 1.54 ± 0.10 in control to 2.33 ± 0.21), wher...
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ورودعنوان ژورنال:
- Cardiovascular research
دوره 77 1 شماره
صفحات -
تاریخ انتشار 2008