Cellulase Activities in Biomass Conversion: Measurement Methods
نویسندگان
چکیده
16 Cellulose, the major constituent of all plant materials and the most abundant 17 organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected 18 by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of 19 hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases) and 20 β-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, 21 enzyme assay is an indispensable part. The most commonly used assays for the 22 individual enzymes as well as total cellulase activity measurements, including their 23
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Cellulase activities in biomass conversion: measurement methods and comparison.
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