Some problems on the leuco-dye-peroxide reaction. The 31st report of histochemical study of peroxidase.

In general, for the histochemical demonstration of peroxidase , b enzidine, naphthol, and leuco-dyes are utilized in the prosence of hydrogen peroxide. Among these , the technical modifications of the benzidine-peroxide method are the most numerous . Alpha-naphthol has been used less often than benzidine . Application of the leucodye method for animal tissues is remarkably limited and this method has been used chiefly for the demonstration of erythrocytes . The present study has dealt with the histochemical application of the leuco-dye method and also with the comparison between diamine-peroxide reaction and leuco-dye-peroxide reaction from the standpoint of histochemical staining .