Transcriptional regulation of the Bacillus thuringiensis subsp. thompsoni crystal protein gene operon.

Transcriptional Regulation of the Bacillus thuringiensis subsp. thompsoni Crystal Protein Gene Operont

Transcriptional regulation of the cryIVD gene operon from Bacillus thuringiensis subsp. israelensis.

The CryIVD protein is involved in the overall toxicity of the Bacillus thuringiensis subsp. israelensis parasporal inclusions and is one of the four major components of the crystals. Determination of the DNA sequence indicated that the cryIVD gene is the second gene of an operon which includes three genes. The first one encodes a 19-kDa polypeptide and has sequence homology with the orf1 gene o...

Unique regulation of crystal protein production in Bacillus thuringiensis subsp. yunnanensis is mediated by the cry protein-encoding 103-megadalton plasmid.

In sporulating cultures of Bacillus thuringiensis subsp. yunnanensis HD977, two cell types are observed: cells forming only spores and cells forming only crystals. Curing analysis suggested that the crystal proteins are plasmid encoded. Through plasmid transfer experiments, it was established that a 103-MDa plasmid is involved in the crystal production. Conjugal transfer of this plasmid to Cry-...

Nucleotide sequence of an additional crystal protein gene cloned from Bacillus thuringiensis subsp. thuringiensis.

The nucleotide sequences of 3 homologous crystal protein genes (cry A1,A2,A3) from Bacillus thuringiensis subsp. kurstaki and subsp. thuringiensis have been reported previously (1, 2, 3, respectively). We report the nucleotide sequence of an additional crystal protein gene {cry hi) from B. thuringiensis subsp. thuringiensis strain HD2 that differs markedly in the aminoterminal region from the 3...

Structural relatedness between mosquitocidal endotoxins of Bacillus thuringiensis subsp. israelensis.

A mosquitocidal toxin gene, cloned from Bacillus thuringiensis subsp. israelensis, was introduced into mutant crystal-negative B. thuringiensis subsp. israelensis cells. Partial toxicity to mosquitos was restored. The 58-kilodalton cloned gene product is a minor protein component of B. thuringiensis subsp. israelensis crystals and is structurally related to a major, 135-kilodalton crystal toxin.