Structural Basis of Transcription: An RNA Polymerase II Elongation
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, 1876 (2001); 292 Science et al. Averell L. Gnatt Complex at 3.3 Å Resolution Structural Basis of Transcription: An RNA Polymerase II Elongation This copy is for your personal, non-commercial use only. clicking here. colleagues, clients, or customers by , you can order high-quality copies for your If you wish to distribute this article to others here. following the guidelines can be obtained by Permission to republish or repurpose articles or portions of articles ): March 10, 2014 www.sciencemag.org (this information is current as of The following resources related to this article are available online at http://www.sciencemag.org/content/292/5523/1876.full.html version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/content/292/5523/1876.full.html#related found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.sciencemag.org/content/292/5523/1876.full.html#ref-list-1 , 13 of which can be accessed free: cites 41 articles This article 414 article(s) on the ISI Web of Science cited by This article has been http://www.sciencemag.org/content/292/5523/1876.full.html#related-urls 100 articles hosted by HighWire Press; see: cited by This article has been http://www.sciencemag.org/cgi/collection/biochem Biochemistry subject collections: This article appears in the following
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Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution.
The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 A resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 3' end of the RNA in the nucleotide addition site. The 3' end is positioned ab...
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A ternary complex composed of RNA polymerase (RNAP), DNA template, and RNA transcript is the central intermediate in the transcription cycle responsible for the elongation of the RNA chain. Although the basic biochemistry of RNAP functioning is well understood, little is known about the underlying structural determinants. The absence of high- resolution structural data has hampered our understa...
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RNA polymerase II (Pol II) is the eukaryotic enzyme that is responsible for transcribing all protein-coding genes into messenger RNA (mRNA). The mRNA-transcription cycle can be divided into three stages: initiation, elongation and termination. During elongation, Pol II moves along a DNA template and synthesizes a complementary RNA chain in a processive manner. X-ray structural analysis has prov...
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The RNA polymerase II elongation is central in eukaryotic transcription. Although multiple intermediates of the elongation complex have been identified, the dynamical mechanisms remain elusive or controversial. Here we build a structure-based kinetic model of a full elongation cycle of polymerase II, taking into account transition rates and conformational changes characterized from both single ...
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