The ABH and Lewis antigens on the human intestinal hydrolases
نویسندگان
چکیده
Lane 1. control jejunal extract; lane 2, A; lane 3, precursor; lane 4. HLe(a-b-); lane 5, Led; lane 6, lacto-N-tetraose; lane 7, lacto-N-fucopentaose I; lane 8,lacto-N-fucopentaose 11; lane 9, lacto-N-difucohexaose I; lane 10, lacto-N-difucohexaose 11; lane I I , lacto-difucotetraose; lane 12. lacto-N-neotetraose; lane 13. 2-fucosyl-lactose; lane 14, 3-fucosyl-lactose. Lanes 2-5 contain ovarian cyst blood group substances. Four other Led substances and one of two HLeh substances also inhibited IG7 binding. One other A substance, one other precursor and one B substance showed little or no inhibition. Titration experiments indicated that the Lea substances were the most potent inhibitors. lacto-N-difucohexaose I I
منابع مشابه
Metabolic and immunologic consequences of ABH secretor and Lewis subtype status.
Determining ABH secretor phenotype and/or Lewis (Le) blood group status can be useful to the metabolically-oriented clinician. For example, differences in ABH secretor status drastically alter the carbohydrates present in body fluids and secretions; this can have profound influence on microbial attachment and persistence. Lewis typing is one genetic marker which might help identify subpopulatio...
متن کاملExpression of mucin peptide and blood group ABH- and Lewis-related carbohydrate antigens in normal human conjunctiva.
PURPOSE The immunohistochemical characterization of mucin peptide antigens and identification of blood group ABH- and Lewis-related carbohydrate antigens expressed in epithelium of normal human conjunctiva. METHODS Immunoperoxidase characterization of conjunctival glycoproteins was performed using antibodies against peptide and saccharide moieties of gastrointestinal mucins on conjunctival bi...
متن کاملExpression of ABH and X (Lex) antigens on platelets and lymphocytes.
We used a panel of reagents, polyclonal and monoclonal antibodies, and lectins to define the expression of the ABH- and Lewis-related specificities on platelets and lymphocytes. We also determined the expression of the alpha 2- and alpha 3-L-fucosyltransferases necessary for their biosynthesis. The antigens that could be detected by immunofluorescence and Western blot analysis were based on typ...
متن کاملThe preparation of biotin-labelled hyaluronidase
Centre and purified using the appropriate Synsorbs (Chembiomed Ltd., Alberta, Canada). Monoclonal anti-A was isolated in the M.R.C. Human Biochemical Genetics Unit. In one experiment lactase from two individuals of different blood group (A and 0) was purified by immunoaffinity chromatography as described in Potter et al. (1985). Using the A blood-group specific antibodies and the lectin from He...
متن کاملABO blood group glycans modulate sialic acid recognition on erythrocytes.
ABH(O) blood group polymorphisms are based on well-known intraspecies variations in structures of neutral blood cell surface glycans in humans and other primates. Whereas natural antibodies against these glycans can act as barriers to blood transfusion and transplantation, the normal functions of this long-standing evolutionary polymorphism remain largely unknown. Although microbial interaction...
متن کامل