Temperature sensitivity of the regulation of nitrogenase synthesis by Klebsiella pneumoniae.
نویسندگان
چکیده
Temperature sensitivity of the regulatory protein coded by nifA prevents the organism from utilizing N2 at 37 degrees C. The purpling of 6-cyanopurine, a function of nifA expression, also is thermolabile.
منابع مشابه
Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis.
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Klebsiella pneumoniae does not fix N(2) under aerobic conditions. The two protein components required for nitrogenase activity were studied during aeration of cells in nitrogen-free media. Component II of nitrogenase was inactivated more slowly in vivo than component I during aeration. The rate of loss of component II was less than the rate of component II synthesis during derepression. No inac...
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In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the phenotypic characterization of nifT and nifT-overexpressing strains for effec...
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متن کاملThe dependence on iron availability of allocation of iron to nitrogenase components in Klebsiella pneumoniae and Escherichia coli.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 145 2 شماره
صفحات -
تاریخ انتشار 1981