Optimization and characterization of invertase by Aspergillus fumigatus using fruit peel waste as substrate
نویسنده
چکیده
The filamentous fungi A. fumigatus produced high levels of invertase when cultured for 96 hr at 30C and pH 5 in Czapek Dox supplemented with fruit peel waste as substrate. Enhanced production occurred on addition of sucrose and yeast extract as nutritional factors. The crude extract was purified to 4.73 fold with recovery of 4.91% by DEAE-column chromatography and the molecular weight was estimated to be 67 KDa by SDS-PAGE. The enzyme activity was stimulated by Na and Ca and inhibited by Zinc. The enzyme hydrolyzed sucrose exhibiting Vmax value of 27.53 U/mg and Km value of 0.28 mg/ml. The activity of the invertase was found to be stable at 50 C for 30 minutes at pH 6.
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