Isolation of glutamine synthetase and glutamotransferase from green peas.

Isolation of Two Quinones with Coenzyme

3. DENES, G. Glutamine synthetase: its stereospecificity and changes induced by activating ions. Biochim. Biophys. Acta 15: 296-297. 1954. 4. DENES, G. and GAZDA, ZS. Untersuchungen iiber die enzymatische Synthese der Siiureamidund Peptidbindung. I. Die enzymatische Synthese von Glutamin in Lupinus albus. Acta Physiol. Acad. Sci. Hung. 4: 1-12. 1953. 5. ELLIOrr, W. H. Studies on the enzymic syn...

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.

Regulation of glutamine synthetase. V. Partial purification and properties of glutamine synthetase from Bacillus licheniformis.

The glutamine synthetase of Bacillus licheniformis has been obtained at about 15% purity. Sucrose gradient centrifugation gave a molecular weight value of approximately 612,000. Both l- and d-glutamate can be utilized as substrates in the biosynthetic reaction, although the l isomer was five times more active. The requirement for adenosine triphosphate (ATP) can be partially replaced by guanosi...

Regulation of glutamine synthetase. VI. Interactions of inhibitors for Bacillus licheniformis glutamine synthetase.

The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP caused synergistic inhibition of glutamine synthesis. Histidine, alanine, and g...

Characterization of Glutamine Synthetase of Roots, Etiolated Cotyledons and Green Leaves from Sinapis alba (L.)

Glutamine synthetase of roots, etiolated cotyledons and green leaves from mustard plants cannot all clearly be separated by DEAE-Sephacel chromatography. However, the enzyme of the roots, etiolated cotyledons and green leaves, respectively, differed in the kinetic properties deter­ mined in the crude extract. The root enzyme showed a pH-optimum of about 6.9, a Km value of 3 m M for glutamate an...