Interaction of heat-denatured HeLa cell DNA with synthetic and natural polysaccharides.
نویسنده
چکیده
Native DNA and polysaccharide (CHO) polymers found in an extract of HeLa cellsl1 as well as the DNA and CHO in sea urchin mitochondria1 preparations12 band a t similar buoyant densities in CsC1. Another association of the two polymers is the interaction of heat-denatured HeLa cell DNA (ADNA) with a CHO from the same kind of cells.3 This interaction may possibly be explained on the basis of hydrogen bonding. If hydrogen bonding accounts for the interaction, CHO polymers other than those isolated from the same source as the DNA should interact with ADNA. This report contains a description of the following properties of HeLa cell DNA: X w , 2 0 values for native and ADNA, melting arid annealing temperatures, rate of melting a t IOO'C, and solubility of ADNA in ethanol. Evidence for the interaction of ADNA with synthetic and natural CHO polymers in 50% ethanol is presented.
منابع مشابه
The interaction of HeLa cell heat denatured DNA with polyglucose.
The aqueous phase in phenol extracts of HeLa cells contain deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and polyglucose (CHO), (Segovia et al. , 1965). -RNA reacts with the CHO and they coprecipitate in 20% ethanol-saline solutions (Graves et al., 1967). -Native DNA precipitates when the ethanol concentration is increased to 50%. Although native DNA and CHO have equal buoyant densities ...
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ورودعنوان ژورنال:
- Biopolymers
دوره 6 11 شماره
صفحات -
تاریخ انتشار 1968