Development and characterization of antisera specific for amino- and carboxy-terminal regions of pp60src.

نویسندگان

  • M D Resh
  • R L Erikson
چکیده

Antisera to the Rous sarcoma virus transforming protein pp60src were produced in rabbits immunized with p60src purified from recombinant Escherichia coli. Immunization with isolated V8 proteolytic fragments of p60src resulted in antisera specific for the amino- and carboxy-terminal domains. The reactions of these antisera with pp60v-src and the normal cell protein p60c-src were characterized.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Immunological, biochemical, and enzymic validation of radioimmunoassays specific to the amino and carboxy terminal of human calcitonin.

We characterized rabbit antisera to synthetic human calcitonin that specifically recognize the aminoand carboxy-terminal regions of calcitonin (antisera 1-506 and HMS-385, respectively), by both classical (parallelism of dose-response curves) and (owing to the unavailability of a variety of analog fragments of human calcitonin) biochemical plus enzymic alteration of synthetic human calcitonin. ...

متن کامل

Antibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen.

Antibodies specific for the amino- and carboxy-terminal portions of simian virus 40 large tumor (T) antigen were obtained by immunization of rabbits with synthetic peptides corresponding to these regions. The amino-terminal synthetic peptide has the sequence Ac-Met-Asp-Lys-Val-Leu-Asn-Arg-(Tyr). The tyrosine residue was introduced in order to couple the peptide to bovine serum albumin with bis-...

متن کامل

Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src.

Transformation of cells by Rous sarcoma virus is mediated by the product of the viral src gene, pp60src. A hybridoma cell line producing an immunoglobulin G3 antibody to pp60src was isolated after lymph node cells from immune mice were fused with mouse myeloma cells (P3-NS1-1). Mice were immunized with p60src purified from Escherichia coli cells expressing the src gene product. The monoclonal a...

متن کامل

Isolation and molecular characterization of partial FSH and LH receptor genes in Arabian camels (Camelus dromedarius)

Very little is known about LHR and FSHR genes of domestic dromedary camels. The main objective of this study was to determine and analyze partial genomic regions of FSHR and LHR genes in dromedary camels for the first time. To this end, a total of 50 DNA samples belonging to dromedary camels raised in Iran were sent for sequencing (25 samples of each gene). We compared the nucleotide sequences ...

متن کامل

Characterization of immunodominant linear B-cell epitopes on the carboxy terminus of the rinderpest virus nucleocapsid protein.

The nucleocapsid (N) protein of rinderpest virus (RPV) is one of the most abundant and immunogenic viral proteins expressed during natural or experimental infection. To identify immunogenic epitopes on the N protein, different forms of RPV N protein, including the full-length protein (N(1-525)), an amino-terminal construct (N(1-179)), and a carboxy-terminal construct (N(414-496)), were expresse...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of virology

دوره 55 1  شماره 

صفحات  -

تاریخ انتشار 1985