Cell separation: Terminology and practical considerations
نویسندگان
چکیده
Cell separation is a powerful tool in biological research. Increasing usage, particularly within the tissue engineering and regenerative medicine communities, means that researchers from a diverse range of backgrounds are utilising cell separation technologies. This review aims to offer potential solutions to cell sorting problems and to clarify common ambiguities in terminology and experimental design. The frequently used cell separation terms of 'purity', 'recovery' and 'viability' are discussed, and attempts are made to reach a consensus view of their sometimes ambiguous meanings. The importance of appropriate experimental design is considered, with aspects such as marker expression, tissue isolation and original cell population analysis discussed. Finally, specific technical issues such as cell clustering, dead cell removal and non-specific antibody binding are considered and potential solutions offered. The solutions offered may provide a starting point to improve the quality of cell separations achieved by both the novice and experienced researcher alike.
منابع مشابه
A suggested Motivational Method for Teaching Scientific Terminology, With a Practical Example
Using a reductionist approach, the motivational method for teaching scientific terminology aims at breaking down terms and their definitions into separate components, i.e. morphemes and their semantic features, rather than establishing a connection between terms and their definitions as holistic units. In other words, the ultimate goal of this method is achieving “semantic motivation,” (as oppo...
متن کاملPractical issues in high-speed cell sorting.
Modern flow cytometric cell sorters are all capable of so-called "high-speed sorting." However, there is confusion about exactly how fast a "high-speed" cell sorter can sort cells. There are many considerations in achieving the fastest sorting speed, as well as the highest quality sort results--cell recovery, purity, and functionality. This requires the same considerations required for "slow-sp...
متن کاملMaking occlusion work: 2. Practical considerations.
In the first of these two articles, occlusal terminology, techniques for examining the occlusion, articulators and interocclusal records were discussed. Here the authors consider some of the practical applications.
متن کاملA Theoretical Review of Disaster’s Social Terminology
Background and Aim: Using common terms and synonyms, such as concepts related to natural crisis managements, is one of the major challenges in interdisciplinary topics; which are apparently similar but have different meanings and practical implications. “Natural disaster” is one of these terms. The main purpose of this study is to describe the common and professional term in science and creatin...
متن کاملPractical Techniques for Improving the Performance of Polymeric Membranes and Processes for Protein Separation and Purification
Protein separation and purification technologies play an essential role in various industries including but not limited to pharmaceuticals, dairy as well as the food sector. Accordingly, a wide variety of techniques such as chromatography and electrophoresis has been developed and utilized extensively over the years for this purpose. Despite their widespread acceptance, conventiona...
متن کامل