Challenges and Opportunities for Small Molecule Aptamer Development

نویسندگان

  • Maureen McKeague
  • Maria C. DeRosa
چکیده

Aptamers are single-stranded oligonucleotides that bind to targets with high affinity and selectivity. Their use as molecular recognition elements has emerged as a viable approach for biosensing, diagnostics, and therapeutics. Despite this potential, relatively few aptamers exist that bind to small molecules. Small molecules are important targets for investigation due to their diverse biological functions as well as their clinical and commercial uses. Novel, effective molecular recognition probes for these compounds are therefore of great interest. This paper will highlight the technical challenges of aptamer development for small molecule targets, as well as the opportunities that exist for their application in biosensing and chemical biology.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Achieving Reproducible Performance of Electrochemical, Folding Aptamer-Based Sensors on Microelectrodes: Challenges and Prospects

Combining specific recognition capabilities with the excellent spatiotemporal resolution of small electrodes represents a promising methodology in bioanalytical and chemical sensing. In this paper, we report the development of reproducible electrochemical, aptamer-based (E-AB) sensors on a gold microelectrode platform. Specifically, we develop microscale sensors (25 μm diameter) for two represe...

متن کامل

East Asian Production Networks – The Role and Contribution of SMEs

The Asia-Pacific region generates over half of global economic activity (54.2%) and about 43.7% of global trade, and is the most dynamic region in the global economy. At the core of this is an increasingly dynamic, vibrant and entrepreneurial small-medium sized enterprise sector. Advances in information and communications technology, market liberalisation and moves towards closer regional econo...

متن کامل

Study of Aptamer-Attached Juglone in Different pH Ranges and Ionic Concentrations of Buffers

    Electrochemical aptamer-based sensors attract a lot of interest as useful methods because of their low cost, accuracy, sensitivity, and simplicity. An electro-active redox molecule comprises the main part of the electrochemical-based sensors. Ferrocene is one of the most popular redox molecule used in biosensor fabrication. But, instability of ferrocenium ion in strong nucleophilic reagents...

متن کامل

Islamic finance for small and medium enterprises: challenges and solutions

Small and medium enterprises (SMEs) play a vital role in the economy. In developing countries, these companies play an important role in creating employment, especially for women. Investing in the development of SMEs is a long-term and smart strategy with sustainable returns. SMEs constitute the vast majority of companies. Globally, SMEs constitute more than 95% of all productive units, when fo...

متن کامل

Radiosensitization of breast cancer cells using AS1411 aptamer-conjugated gold nanoparticles

Introduction: A main choice for cancer treatment is radiotherapy. But, the radiotherapy disadvantage is damages caused by radiation given to normal tissues/organs surrounding cancer. One way to avoid this is via increasing radiosensitization of cancer cells. Gold nanoparticles (GNPs) have shown sensitizing effect on cancer cells by enhancing their absorbed dose. Unlike earlier ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2012  شماره 

صفحات  -

تاریخ انتشار 2012