Nuclease tolerant FRET probe based on DNA-quantum dot conjugation.
نویسندگان
چکیده
We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).
منابع مشابه
Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense
An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen. Modified QD that contained carboxylic groups was conjugated with a single-stranded DNA probe (ssDNA) via amide-linkage. Hybridization of the target DNA with conjugate...
متن کاملDNA mediated assembly of quantum dot-protoporphyrin IX FRET probes and the effect of FRET efficiency on ROS generation.
Photodynamic therapy (PDT) involves generation of reactive oxygen species (ROS) by the irradiation of a photosensitizer. Controlled and targeted release of ROS by a photosensitizer is crucial in PDT. For achieving controlled generation of ROS, a ZnSe/ZnS quantum dot (QD) donor and protoporphyrin IX (Pp IX) acceptor based fluorescence resonance energy transfer (FRET) probe is reported here. The ...
متن کاملCdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids.
We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluo...
متن کاملFault-tolerant adder design in quantum-dot cellular automata
Quantum-dot cellular automata (QCA) are an emerging technology and a possible alternative for faster speed, smaller size, and low power consumption than semiconductor transistor based technologies. Previously, adder designs based on conventional designs were examined for implementation with QCA technology. This paper utilizes the QCA characteristics to design a fault-tolerant adder that is more...
متن کاملFault-tolerant adder design in quantum-dot cellular automata
Quantum-dot cellular automata (QCA) are an emerging technology and a possible alternative for faster speed, smaller size, and low power consumption than semiconductor transistor based technologies. Previously, adder designs based on conventional designs were examined for implementation with QCA technology. This paper utilizes the QCA characteristics to design a fault-tolerant adder that is more...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 24 2 شماره
صفحات -
تاریخ انتشار 2008