Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi.

Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.

Two different strains, Aspergillus awamori TGDTh-4 and A. awamori TGP-3 overexpressing a synthetic gene encoding the plant sweet protein thaumatin, showed an unfolded protein response. To facilitate protein secretion, the chaperone BiPA gene was expressed in A. awamori under control of the strong constitutive promoter of the gpdA gene. A good correlation was observed between the level of the bi...

Molecular Cloning of the Glucoamylase I Gene of AspergMus awamori var . kawachi for Localization of the Raw - starch - aMnity Site

The development of Aspergillus niger var. awamori as a host for the expression and secretion of heterologous gene products.

Filamentous fungi possess unique features which make them attractive as hosts for the production of heterologous gene products. For example, certain fungal species are capable of secreting large quantities of protein in submerged culture. Selected strains of Aspergillus niger can produce greater than 20 g of glucoamylase I ~ I in industrial fermentations [ 11. Some filamentous fungi, including ...

Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution.

The three-dimensional structure of the pseudotetrasaccharide acarbose complexed with glucoamylase II(471) from Aspergillus awamori var. X100 has been determined to 2.4-A resolution. The model includes residues corresponding to 1-471 of glucoamylase I from Aspergillus niger, a single molecule of bound acarbose, and 535 sites for water molecules. The crystallographic R factor from refinement is 0...

Predicted unfolding order of the 13 alpha-helices in the catalytic domain of glucoamylase from Aspergillus awamori var. X100 by molecular dynamics simulations.

The unfolding mechanism of the 13 alpha-helices in the catalytic domain of Aspergillus awamori var. X100 glucoamylase was investigated by 200 ps molecular dynamics simulations in explicit water with temperature jump technique. Rather than a simultaneous event, the unfolding of these 13 alpha-helices followed a random ordered mechanism as alpha8-->alpha1-->alpha11-->alpha7-->alpha10-->alpha3-->a...