Polygalacturonase in normal and abnormal tomato fruit.
نویسنده
چکیده
منابع مشابه
Degradation of isolated tomato cell walls by purified polygalacturonase in vitro.
Cell wall preparations from green pericarp of normal and mutant Neverripe (Nr) and ripening inhibitor (rin) tomato (Lycopersicon esculentum Mill.) fruit were all equally degraded in vitro by a cell wall-bound protein extract from ripe normal tomatoes.Similar cell wall-bound protein extracts from ripe Nr fruit were not as effective and those from ripe rin fruit gave no cell wall degradation at a...
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Tomato fruit ripening is accompanied by extensive degradation of pectic cell wall components. This is thought to be due to the action of a single enzyme, polygalacturonase, whose activity is controlled, at least in part, at the level of gene expression. At the onset of tomato fruit ripening, polygalacturonase enzyme activity, mRNA levels, and relative rate of gene transcription all increase dra...
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Although the pectin content of tomatoes is relatively low in comparison with other fruits (Money & Christian, 1950), the activity of pectinesterase (pectin pectyl-hydrolase, EC 3.1.1.11) is particularly high (Kertesz, 1938). Softening of the fruit takes place relatively rapidly during ripening (Hobson, 1959), and the classical mechanism by which the effect is usually explained assumes the solub...
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Fruit ripening is a coordinated series of biochemical changes that renders the fruit attractive to eat. The fruit may soften, develop colour, change starch or acid into sugar, become flavoursome, produce more ethylene, increase in sensitivity to ethylene, and respire more rapidly. This syndrome is under contemporary genetic control as illustrated by mutants with fruit that develop normally but ...
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Tomato polygalacturonase is a cell wall enzyme secreted in large amounts during tomato fruit ripening. Polygalacturonase is synthesized as a glycoprotein precursor that undergoes numerous cotranslational and post-translational processing steps during its maturation, yielding three isozymes in tomato fruit, PG1, PG2A, and PG2B. To investigate the physiological roles of the three isozymes and the...
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عنوان ژورنال:
- The Biochemical journal
دوره 92 2 شماره
صفحات -
تاریخ انتشار 1964