Antibacterial, Phytochemical and Antioxidant activities of Datura metel

The antibacterial efficacy of crude aqueous and ethanol extracts leaf, stem bark and roots of Datura metel at 20mg/ml were assayed against eight clinical bacterial isolates (Streptococcus b hemolytic, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus cereus and Streptococcus dysenteriae) by well in agar method The leaf and stem bark of the plant was antagonistic against the test bacteria species with inhibitory zones of between 35-12 mm while the highest inhibitory zone displayed by one of the reference drug (streptomycin) was 45 mm at 10mg/ml concentration. Among the tested bacterial isolates Staph. aureus was the most inhibited majorly with the ethanol extract. The aqueous extract displayed inhibitory zones of between 22-10 mm while the crude ethanol extract exhibited inhibitory affinity between 32-12 mm. The MIC of the plant extracts on the susceptible bacterial isolates was at 20mg/ml and MBC affinity was at 20-10mg/ml. The aqueous extracts of the plants was more sufficient in the extraction of the substances with phytochemical and antioxidant activities than ethanol extract. The phytochemical compounds common among the crude aqueous and ethanol extracts of the plant parts were saponins, flavonoids, alkaloids, glycocides and phenol. The aqueous extract of the plant displayed antioxidant activity of between 49.3023.82% while the ethanol extract was between 25.51-3.41%. The general results observed consider the plant as natural source of antioxidants and phytochemical quality for antimicrobial effectiveness.