Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization.

A plaque reduction test for dengue virus neutralizing antibodies.

The need for an efficient, practical, and accurate laboratory method for measuring dengue virus neutralizing antibody has been recognized by virologists concerned with arboviruses. Methods previously used include measurement of log neutralization indices by serial virus dilution tests with constant serum in suckling mice (1) or tissue culture tubes (2), or measurement of serum titers in sucklin...

Improved Dengue Virus Plaque Formation on BHK21 and LLCMK2 Cells: Evaluation of Some Factors

[email protected]; " 53-7-2020450; Fax: 53-7-2046051 Neutralizing antibodies play a key role in the prevention of dengue infection. It is important that dengue virus plaque titration and plaque reduction neutralization tests (PRNT) be highly reproducible using standardized methods. To evaluate factors that have influence in the PRNT for dengue viruses, neutralizing antibodies were determined in 2...

Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization.

A newly modified semimicro plaque reduction neutralization test (PRNT) in BHK cells was compared with a standard PRNT in bottles with LLC-MK2 monolayers and with an LLC-MK2 PRNT adapted to semimicro methods. The BHK semimicro PRNT compared favorably in terms of sensitivity in detecting dengue antibody (96%), specificity at a screening dilution (95%), and ability to detect seroconversion to deng...

Flow cytometry-based assay for titrating dengue virus.

Plaque assays for titrating dengue virus (DENV) are time-consuming and not suitable for strains that do not plaque. Fluorescence-activated cell sorting (FACS) has been used to detect DENV-infected cells. Here we describe a FACS-based assay for titrating DENV. We determined that at 24 h postinfection, the number of infected cells detected by FACS represented the first round of infection and ther...

Development of Plaque Techniques for Titration and Neutralization Tests with African Horse-Sickness Virus