The qualitative analysis of ethanol extracts of herbal raw materials by method of high pressure liquid chromatography.

The article describes the qualitative analysis of ethanol extracts from St. John's wort, cinnamon bark, rosemary leaves, lavender flowers, and ginger rhizomes, and the extract of their mixture using the method of high pressure liquid chromatography in order to identify flavonoids. The aim of the study was to identify the amounts of rutin, hyperoside, quercetin, and other flavonoids in the multi-component extract as well as in the control extracts of its compositional parts using the method of high pressure liquid chromatography, by evaluation of the correlation between the amounts of flavonoids in the extracts. The evaluation of the results of the study showed that flavonoid amount in the control extracts of the herbal material and flavonoid amount in the ethanol extracts of the mixture of raw material did not differ significantly. It was found that the largest amount of flavonoids was extracted from St. John's wort. The results of the analysis confirmed the assumption that the yield of flavonoids from St. John's wort has no influence on the active substance of other medicinal herbal raw materials.